The PI(4,5)P2 Mass ELISA detects the amount PI(4,5)P2 extracted from cells by means of a competitive 96-well ELISA assay. Assay Range: 250 to 0.016 pmol PI(4,5)P2 Sample Type: Dried down lipid extracted from cells Sample Volume: 180 µL/sample, run in triplicate
Product Description The PI(4,5)P2 Mass ELISA assay is a competitive ELISA in which the signal is inversely proportional to the amount of PI(4,5)P2 produced. Once PI(4,5)P2 has been extracted from cell samples, it is incubated with a PI(4,5)P2 detector protein, then added to the PI(4,5)P2-coated microplate for competitive binding. A peroxidase-linked secondary detection reagent and colorimetric substrate is used to detect the PI(4,5)P2 detector protein binding to the plate. The colorimetric signal is inversely proportional to the amount of PI(4,5)P2 extracted from cells. Phosphatidylinositol 4,5-bisphosphate, or PI(4,5)P2, is a ubiquitous lipid shown to play a central role in a variety of cell functions including the PKB/Akt pathway leading to cell growth, motility, inflammation, and apoptosis. PI(4,5)P2 is a substrate for class I phosphoinositide 3-kinase (PI3-K) and the product of PTEN phosphatase activity. Featured in Publications 1. Musse, A.A., Gao, W. et al. Myelin basic protein co-distributes with other PI(4,5)P2-sequestering proteins in Triton X-100 detergent-resistant membrane microdomains. Neuroscience Letters, 450(1):32-36 2. Costa, C., Ebi, H., et al. Measurement of PIP3 Levels Reveals an Unexpected Role for p110beta in Early Adaptive Responses to p110alpha-Specific Inhibitors in Luminal Breast Cancer Cell 27(1):97-108 3. Gross, C., C.-W. Chang, et al. (2015). Increased Expression of the PI3K Enhancer PIKE Mediates Deficits in Synaptic Plasticity and Behavior in Fragile X Syndrome. Cell Reports, DOI:10.1016/j.celrep.2015.03.060 4. S. Choi, et al. Agonist-stimulated phosphatidylinositol-3,4,5-trisphosphate generation by scaffolded phosphoinositide kinases Nature Cell Biology (2016) 18(12), 1324-35, doi:10.1038/ncb3441 5. Reed, D. E. and K. M. Shokat (2017). INPP4B and PTEN Loss Leads to PI-3,4-P2 Accumulation and Inhibition of PI3K in TNBC. Mol Cancer Res 15(6): 765-775. 6. Hawse, W. F. and R. T. Cattley (2019). T cells transduce T-cell receptor signal strength by generating different phosphatidylinositols. J Biol Chem 294(13): 4793-4805. Keywords: PI3 Kinase, PI3 K, PIP2, PTEN, Phosphatidylinositol (4,5)-bisphosphate, PtdIns(4,5)P2, PI(4,5)P2, PIP2, Phosphatidylinositol 3-kinase, PI 3-kinase, PI3-Kinase, PI3-K, PI3K, PTEN, Phosphatase and tensin homolog, Phosphatases, cell extraction, lipid extraction, extraction
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