PI(3,5)P2 PIP Beads
Catalog Number:
ECH-P-B035A
Product Overview
Article Name:
PI(3,5)P2 PIP Beads
Biozol Catalog Number:
ECH-P-B035A
Supplier Catalog Number:
P-B035A
Alternative Catalog Number:
ECH-P-B035A-100UL, ECH-P-B035A-1ML
Manufacturer:
Echelon Biosciences
Category:
Molekularbiologie
Product Description
PI(3,5)P2 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(3,5)P2 per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase. Storage 4 C - do not freeze Applications PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis. Featured in Publications 1) Dong, X.-p., D. Shen, et al. (2010). PI(3,5)P2 controls membrane trafficking by direct activation of mucolipin Ca2+ release channels in the endolysosome. Nat Commun 1(4): 38. 2) Galvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039. 3) Li, X., et al. (2013). Genetically encoded fluorescent probe to visualize intracellular phosphatidylinositol 3,5-bisphosphate localization and dynamics. Proc Natl Acad Sci U S A 110(52): 21165-21170. Being bio-reactive blog: Lipid-Ligand interaction tools: lipids are not scary intermediates anymore Documents Technical Data Sheet
Application Notes
Application Notes:
Categories: Biochemical Reagents. Related: Beads, Phosphoinositides, PI(3\,5)P2. Ship 1 : Shipping Temp. Ship 2: Gel Ice
