PI(3,4,5)P3 PIP Beads
Catalog Number:
ECH-P-B345A
Product Overview
Article Name:
PI(3,4,5)P3 PIP Beads
Biozol Catalog Number:
ECH-P-B345A
Supplier Catalog Number:
P-B345A
Alternative Catalog Number:
ECH-P-B345A-100UL,ECH-P-B345A-1ML
Manufacturer:
Echelon Biosciences
Category:
Molekularbiologie
Product Description
PI(3,4,5)P3 PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(3,4,5)P3 per mL of beads, enough for several protein binding experiment. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase. Storage 4 C - do not freeze Applications PI(3,4,5)P3 PIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis. Featured in Publications 1) Hiromura, M., F. Okada, et al. (2004). Inhibition of Akt kinase activity by a peptide spanning the betaA strand of the proto-oncogene TCL1. J Biol Chem 279(51): 53407-18. 2) Craig, H. E., J. Coadwell, et al. (2010). ARAP3 binding to phosphatidylinositol-(3,4,5)-trisphosphate depends on N-terminal tandem PH domains and adjacent sequences. Cellular Signalling 22(2): 257-264. 3) Takahashi, K. and K. Suzuki (2010). WAVE2 targeting to phosphatidylinositol 3,4,5-triphosphate mediated by insulin receptor substrate p53 through a complex with WAVE2. Cellular Signalling 22(11): 1708-1716. 4) Galvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039. http://www.bio-protocol.org/e1039 5) Barrows, D., et al. (2016). PREX1 Protein Function is Negatively Regulated Downstream of Receptor Tyrosine Kinase Activation by p21-activated Kinases (PAKs). The Journal of biological chemistry 291: 20042-20054. 6) Lin, A., et al. (2017). The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors. Nat Cell Biol 19(3): 238-251. 7) Hawse, W. F. and R. T. Cattley (2019). T cells transduce T-cell receptor signal strength by generating different phosphatidylinositols. J Biol Chem 294(13): 4793-4805. Being bio-reactive blog: Lipid-Ligand interaction tools: lipids are not scary intermediates anymore Documents Technical Data Sheet
Application Notes
Application Notes:
Categories: Biochemical Reagents. Related: Beads, Phosphoinositides, PI(3\,4\,5)P3. Ship 1 : Shipping Temp. Ship 2: Gel Ice
