The Collagen Hybridizing Peptides are products of 3-Helix Inc. The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo [1,2,3]. By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature. CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging [3]. CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells [4]. CHP can measure and localize mechanical injury to collagenous tissue at the molecular level [5]. It also enables assessment of collagen denaturation in decellularized extracellular matrix [6]. In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot [7]. CHP come in three varieties: labeled with biotin (Catalog : BIO) for avidin/streptavidin-mediated including HRP-enzyme methods, 5-FAM (Catalog : FLU) for green fluorescence detection, and Cy3 (Catalog : RED) for red fluorescence detection. Applications: immunofluorescence, immunohistochemistry, SDS-PAGE (in-gel Western blot) Shipping Conditions Express shipping as powder at ambient temperature. Store at -20 C upon arrival until use. Storage -20 C as powder, 4 C after reconstitution in water, no need to aliquot and freeze. References 1) ''Targeting and mimicking collagens via triple helical peptide assemblies Curr Opin Chem Biol, 2013, 17, 968-975. 2) ''Targeting collagen strand by photo-triggered triple-helix hybridization Proceedings of the National Academy of Sciences of the United States of America, 2012, 109, 14767-14772. DOI: 10.1073/pnas.1209721109. 3) In situ imaging of tissue remodeling with collagen hybridizing peptides. ACS Nano, 2017, 11, 9825. DOI: 10.1021/acsnano.7b03150 4) Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging. Biomaterials, 2018, DOI:10.1016/j.biomaterials.2018.08.039 5) Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides. Nat. Commun., 2017, 8, 14913, DOI: 10.1038/ncomms14913. 6) Molecular assessment of collagen denaturation in decellularized tissues using the collagen hybridizing peptide. Acta Biomater., 2017. 53, 268-278, DOI: 10.1016/j.actbio.2017.01.079 7) ''Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides Bioconjugate Chemistry, 2013, 24, 9-16. DOI: 10.1021/bc3005842. 8) In tendons, differing physiological requirements lead to functionally distinct nanostructures. Sci. Reports, 2018. DOI: DOI:10.1038/s41598-018-22741-8 Features -More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM -High affinity and unparalle |
