Control PolyPIPosomes

Catalog Number: ECH-Y-0000
Article Name: Control PolyPIPosomes
Biozol Catalog Number: ECH-Y-0000
Supplier Catalog Number: Y-0000
Alternative Catalog Number: ECH-Y-0000-100UL,ECH-Y-0000-250UL
Manufacturer: Echelon Biosciences
Category: Molekularbiologie
PolyPIPosomes are polymerized liposomes that show markedly increased stability (up to 6 months) vs. conventional liposomes. A biotin tag is incorporated for easy detection of PIPn-binding proteins with streptavidin reagents. - Uniform size (~200 nm) - Ready-to-use suspensions, no liposome preparation PolyPIPosomes have demonstrated utility in a number of assay formats: -Protein pull-down -Surface Plasmon Resonance -Liposome overlay assay
Control PolyPIPosomes are provided as a 1mM total lipid solution without any PIPn for use as a negative control to confirm there is no non-specific binding to the liposomes.

Featured in Publications

1) Ferguson, C. G., R. D. James, et al. (2005). Phosphoinositide-Containing Polymerized Liposomes: Stable Membrane-Mimetic Vesicles for Protein-Lipid Binding Analysis. Bioconjug Chem 16(6): 1475-1483. 2) Elkin, S. K., D. Ivanov, et al. (2005). A PHD finger motif in the C-terminus of RAG2 modulates recombination activity. J Biol Chem 280(31): 28701-10. 3) Mustafa, A. K., D. B. van Rossum, et al. (2009). Glutamatergic regulation of serine racemase via reversal of PIP2 inhibition. Proc Natl Acad Sci U S A 106(8): 2921-6. 4) Zemskov, E. A., I. Mikhailenko, et al. (2011). Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes. PLoS One 6(4): e19414. Being bio-reactive blog: Lipid-Ligand interaction tools: lipids are not scary intermediates anymore

Documents

Technical Data Sheet
Application Notes: Categories: Biochemical Reagents. Related: Phosphoinositides, PolyPIPosomes. Ship 1 : Shipping Temp. Ship 2: Gel Ice