DNA-PKcs (16N19) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE10074
Product Overview
Article Name:
DNA-PKcs (16N19) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE10074
Supplier Catalog Number:
AMRe10074
Alternative Catalog Number:
EKL-AMRE10074-50UL, EKL-AMRE10074-100UL, EKL-AMRE10074-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
ICC, IHC, WB
Species Reactivity:
Human, Mouse, Rat
Conjugation:
Unconjugated
Alternative Names:
DNA-PKcs, DNA-dependent protein kinase catalytic subunit, DNPK1, EC 2.7.11.1, P460, PRKD, PRKDC, XRCC7, kinase DNA PK, DNA PKcs,
Product Description
DNA-dependent protein kinase (DNA-PK) is an important factor in the repair of double-stranded breaks in DNA. Cells lacking DNA-PK or in which DNA-PK is inhibited fail to show proper nonhomologous end-joining (NHEJ). DNA-PK is composed of two DNA-binding subunits (Ku70 and Ku86) and one 450 kDa catalytic subunit (DNA-PKcs). It is thought that a heterodimer of Ku70 and Ku86 binds to double-stranded DNA broken ends before DNA-PKcs binds and is activated. Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage (PubMed:11955432, PubMed: 12649176, PubMed: 14734805, PubMed: 33854234). Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination (PubMed: 11955432, PubMed: 12649176, PubMed: 14734805, PubMed: 33854234). Must be bound to DNA to express its catalytic properties (PubMed: 11955432). Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C) (PubMed: 11955432). Recruited by XRCC5 and XRCC6 to DNA ends and is required to (1) protect and align broken ends of DNA, thereby preventing their degradation, (2) and sequester the DSB for repair by NHEJ (PubMed: 15574326, PubMed: 11955432, PubMed: 12649176, PubMed: 14734805, PubMed: 33854234). Act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage (PubMed: 15574326, PubMed: 11955432, PubMed: 12649176, PubMed: 14734805). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step (PubMed: 15574326, PubMed: 11955432, PubMed: 12649176, PubMed: 14734805). Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion (By similarity). Also involved in modulation of transcription (PubMed: 15574326, PubMed: 11955432, PubMed: 12649176, PubMed: 14734805). As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small
Product Properties
Clonality:
Monoclonal
Molecular Weight:
469kDa
NCBI:
5591
UniProt:
P78527
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
PRKDC
Application Notes
Application Dilute:
WB 1:1000-1:5000,IHC 1:50-1:100,ICC/IF 1:100-1:200
