GRP78 BiP (11J12) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE11793
Product Overview
Article Name:
GRP78 BiP (11J12) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE11793
Supplier Catalog Number:
AMRe11793
Alternative Catalog Number:
EKL-AMRE11793-50UL, EKL-AMRE11793-100UL, EKL-AMRE11793-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
FC, IF, IHC, WB
Species Reactivity:
Human, Mouse, Rat
Conjugation:
Unconjugated
Alternative Names:
GRP-78, GRP78, BIP, MIF2, HSPA5
Product Description
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as immunoglobulin heavy chain-binding protein (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:2294010, PubMed: 23769672, PubMed: 23990668, PubMed: 28332555). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed: 1550958, PubMed: 19538957). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation (PubMed: 26045166).
Product Properties
Clonality:
Monoclonal
Molecular Weight:
72kDa
NCBI:
3309
UniProt:
P11021
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
HSPA5
Application Notes
Application Dilute:
WB 1:1000-1:5000,IHC 1:100-1:500,FC 1:100-1:200,IF-P 1:100-1:500
