HLA-DRA (4K6) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE12087
Product Overview
Article Name:
HLA-DRA (4K6) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE12087
Supplier Catalog Number:
AMRe12087
Alternative Catalog Number:
EKL-AMRE12087-50UL, EKL-AMRE12087-100UL, EKL-AMRE12087-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
FC, ICC, IHC, WB
Species Reactivity:
Human
Conjugation:
Unconjugated
Alternative Names:
HLA-DR, MLRW, HLA-DRA1, MHC class II antigen DRA, HLA class II histocompatibility antigen, DR alpha chain, HLA DRB1, DRA,
Product Description
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA- DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T- helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells (PubMed:29884618, PubMed: 17334368, PubMed: 8145819, PubMed: 15322540, PubMed: 22327072, PubMed: 27591323, PubMed: 31495665, PubMed: 15265931, PubMed: 9075930, PubMed: 24190431). Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes (PubMed: 8145819). In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins (PubMed: 31495665). Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance (PubMed: 17182262, PubMed: 23783831). The selection of the immunodominant epitopes follows two processing modes: bind first, cut/trim later for pathogen-derived antigenic peptides and cut first, bind later for autoantigens/self- peptides (PubMed: 25413013). The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules (PubMed: 8145819).
Product Properties
Clonality:
Monoclonal
Molecular Weight:
29kDa
NCBI:
3122
UniProt:
P01903
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
HLA-DRA
Application Notes
Application Dilute:
WB 1:1000-1:5000,IHC 1:100-1:1000,ICC/IF 1:200-1:500,FC 1:20-1:50
