PRMT5 (16R15) Rabbit Monoclonal Antibody, Unconjugated

Product Overview

• Article Name: PRMT5 (16R15) Rabbit Monoclonal Antibody, Unconjugated
• Biozol Catalog Number: EKL-AMRE16505
• Supplier Catalog Number: AMRe16505
• Alternative Catalog Number: EKL-AMRE16505-50UL, EKL-AMRE16505-100UL, EKL-AMRE16505-200UL
• Manufacturer: EnkiLife
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs, PRMT5, Skb1Hs Methyltransferase,

Product Description

Involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p. An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast two-hybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK). Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA (PubMed:10531356, PubMed:11152681, PubMed:11747828, PubMed:12411503, PubMed:15737618, PubMed:17709427, PubMed:20159986, PubMed:20810653, PubMed:21258366, PubMed:21917714, PubMed:22269951, PubMed:21081503). Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), such methylation being required for the assembly and biogenesis of snRNP core particles (PubMed:12411503, PubMed:11747828, PubMed:17709427). Methylates SUPT5H and may regulate its transcriptional elongation properties (PubMed:12718890). Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 Arg-3 during germ cell development (By similarity). Methylates histone H3 Arg-8, which may repress transcription (By similarity). Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (By similarity). Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR, this enhances EGFR Tyr-1197 phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation (PubMed:21917714, PubMed:21258366). Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity (PubMed:21917714). Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9 (PubMed:22269951). Methylates and regulates SRGAP2 which is involved in cell migration and differentiation (PubMed:20810653). Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulati

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 73kDa
• NCBI: 10419
• UniProt: O14744
• Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
• Purity: Affinity purification
• Form: Liquid
• Target: PRMT5

Application Notes

• Application Dilute: WB 1:2000-1:20000,IHC 1:50-1:100,ICC/IF 1:20-1:50,FC 1:50-1:200,IP 1:20-1:50