Bovine IGFBP4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit
Biozol Catalog Number:
EKL-EBV27398
Supplier Catalog Number:
EBv27398
Alternative Catalog Number:
EKL-EBV27398-96T
Manufacturer:
EnkiLife
Category:
Kits/Assays
Species Reactivity:
Bovine
Alternative Names:
BP4, HT29-IGFBP, IBP4
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine IGFBP4. During the reaction, Bovine IGFBP4 in the sample or standard competes with a fixed amount of Bovine IGFBP4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Bovine IGFBP4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Bovine IGFBP4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Range:
74.07-6000pg/mL
Sensitivity:
29.62pg/mL
Samples:
Serum,plasma and other biological fluids
Target:
IGFBP4
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