Bovine IGFBP2 (Insulin Like Growth Factor Binding Protein 2) ELISA Kit
Biozol Catalog Number:
EKL-EBV27400
Supplier Catalog Number:
EBv27400
Alternative Catalog Number:
EKL-EBV27400-96T
Manufacturer:
EnkiLife
Category:
Kits/Assays
Species Reactivity:
Bovine
Alternative Names:
IBP2, IGF-BP53
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Bovine IGFBP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Bovine IGFBP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Bovine IGFBP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Bovine IGFBP2. You can calculate the concentration of Bovine IGFBP2 in the samples by comparing the OD of the samples to the standard curve.
Range:
0.94-60ng/mL
Sensitivity:
0.32ng/mL
Samples:
Serum,plasma and other biological fluids
Target:
IGFBP2
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