Human COL1alpha1 (Collagen Type 1 Alpha 1) ELISA Kit
Biozol Catalog Number:
EKL-EH10056
Supplier Catalog Number:
EH10056
Alternative Catalog Number:
EKL-EH10056-96T
Manufacturer:
EnkiLife
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
COL1A1, EDSC, OI1, OI2, OI3, OI4, collagen type I alpha 1, collagen type I alpha 1 chain, EDSARTH1
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL1alpha1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL1alpha1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL1alpha1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Human COL1alpha1. You can calculate the concentration of Human COL1alpha1 in the samples by comparing the OD of the samples to the standard curve.
