Human NEFL (Neurofilament, Light Polypeptide) ELISA Kit
Biozol Catalog Number:
EKL-EH25189
Supplier Catalog Number:
EH25189
Alternative Catalog Number:
EKL-EH25189-96T
Manufacturer:
EnkiLife
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
CMT1F, CMT2E, NF-L, NF68, NFL, 68 kDa neurofilament protein, Neurofilament triplet L protein
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human NEFL. During the reaction, Human NEFL in the sample or standard competes with a fixed amount of Human NEFL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human NEFL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Human NEFL in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Range:
123.5-10000pg/mL
Sensitivity:
49.3pg/mL
Samples:
Serum,plasma and other biological fluids
Target:
NEFL
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