Human ProNT/NMN (Proneurotensin/neuromedin N) ELISA Kit
Biozol Catalog Number:
EKL-EH25225
Supplier Catalog Number:
EH25225
Alternative Catalog Number:
EKL-EH25225-96T
Manufacturer:
EnkiLife
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
Proneurotensin 1-117
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human ProNT/NMN. During the reaction, Human ProNT/NMN in the sample or standard competes with a fixed amount of Human ProNT/NMN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ProNT/NMN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Human ProNT/NMN in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Range:
12.35-1000pg/mL
Sensitivity:
4.84pg/mL
Samples:
Serum,plasma and other biological fluids
Target:
ProNT/NMN
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