a-CTXI, aCTX-I, A-Cr, Type I Collagen C-Telopeptide-Related Fraction
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse alpha-CTx. During the reaction, Mouse alpha-CTx in the sample or standard competes with a fixed amount of Mouse alpha-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse alpha-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Mouse alpha-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Range:
74.07-6000pg/mL
Sensitivity:
26.39pg/mL
Samples:
Serum,plasma and other biological fluids
Target:
alpha-CTx
* VAT and and shipping costs not included. Errors and price changes excepted
