Rat MOTS-c (Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit
Biozol Catalog Number:
EKL-ER21519
Supplier Catalog Number:
ER21519
Alternative Catalog Number:
EKL-ER21519-96T
Manufacturer:
EnkiLife
Category:
Kits/Assays
Species Reactivity:
Rat
Alternative Names:
MOTS-c
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat MOTS-c. During the reaction, Rat MOTS-c in the sample or standard competes with a fixed amount of Rat MOTS-c on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat MOTS-c. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Rat MOTS-c in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Range:
0.247-20ng/mL
Sensitivity:
0.108ng/mL
Samples:
Serum,plasma and other biological fluids
Target:
MOTS-c
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