Rat CD11a (Lymphocyte Function Associated Antigen 1 Alpha) ELISA Kit
Biozol Catalog Number:
EKL-ER21839
Supplier Catalog Number:
ER21839
Alternative Catalog Number:
EKL-ER21839-96T
Manufacturer:
EnkiLife
Category:
Kits/Assays
Species Reactivity:
Rat
Alternative Names:
LFA1a, ITG-AL, LFA-1, LFA1-A, ITGAL, Integrin,Alpha L, CD11 antigen-like family member A, Leukocyte function-associated molecule 1 alpha chain
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CD11a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CD11a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CD11a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat CD11a. You can calculate the concentration of Rat CD11a in the samples by comparing the OD of the samples to the standard curve.
Range:
0.15-10ng/mL
Sensitivity:
0.055ng/mL
Samples:
Serum,plasma and other biological fluids
Target:
CD11a
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