Rat SPA2 (Surfactant Associated Protein A2) ELISA Kit
Biozol Catalog Number:
EKL-ER22202
Supplier Catalog Number:
ER22202
Alternative Catalog Number:
EKL-ER22202-96T
Manufacturer:
EnkiLife
Category:
Kits/Assays
Species Reactivity:
Rat
Alternative Names:
SFTPA2B, COLEC5, SP2A, SPA1 beta, SP-A2, SPAII, Collectin-5, Pulmonary Surfactant Protein A2, 35 kDa pulmonary surfactant-associated protein, Alveolar proteinosis protein
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SPA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SPA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SPA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat SPA2. You can calculate the concentration of Rat SPA2 in the samples by comparing the OD of the samples to the standard curve.
Range:
0.15-10ng/mL
Sensitivity:
0.062ng/mL
Samples:
Serum,plasma and other biological fluids
Target:
SPA2
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