This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 5-Me-THF. During the reaction, 5-Me-THF in the sample or standard competes with a fixed amount of 5-Me-THF on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 5-Me-THF. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of 5-Me-THF in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Range:
1.11-90ng/mL
Sensitivity:
0.42ng/mL
Samples:
Serum,plasma and other biological fluids
Target:
5-Me-THF
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