Recombinant Rat TNF alpha, E. coli

Product Overview

• Article Name: Recombinant Rat TNF alpha, E. coli
• Biozol Catalog Number: EKL-PER1678
• Supplier Catalog Number: PER1678
• Alternative Catalog Number: EKL-PER1678-10UG,EKL-PER1678-50UG,EKL-PER1678-500UG
• Manufacturer: EnkiLife
• Host: E. coli
• Category: Proteine/Peptide
• Species Reactivity: Rat
• Alternative Names: Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, Tumor Necrosis Factor, Membrane Form, Tumor Necrosis Factor, Soluble Form, Tnf, Tnfa, Tnfsf2

Product Description

Tumor necrosis factor alpha (TNF-alpha, TNFSF2) is the prototypic ligand of the TNF superfamily. Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.

Product Properties

• Molecular Weight: 17.4 KDa
• NCBI: 16599
• Purity: Greater than 95% as determined by reducing SDS-PAGE

Application Notes

• Application Notes: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100µg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.