Liquid. In TRIS-HCl, pH 7.4, 150mM sodium chloride, 1mM magnesium chloride and 10% (v/v) glycerol.
Target:
5-nucleotidase
Application Notes:
5-nucleotidase is useful as the second enzyme in coupled assays of phosphodiesterases (3,5-cyclic nucleotide phosphodiesterase, EC 3.1.4.17). Phosphodiesterase catalyzed hydrolysis of, for example, cAMP to 5-AMP is coupled to 5-nucleotidase cleavage
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