Produced in E. coli. Human calcineurin Aalpha (MW 60kDa) and calcineurin B (MW 15kDa) heterodimer. Both subunits are coexpressed in a construct with yeast myristoyl-CoA:protein N-myristoyltransferase. The resulting highly active calcineurin (protein phosp
Purity:
90% (SDS-PAGE)
Form:
Liquid. In 1X KI-128 Assay Buffer with increased DTT concentration (50mM TRIS, pH 7.5, containing 100mM sodium chloride, 6mM MgCl2, 5mM DTT, 0.025% NP-40 and 0.5mM CaCl2).
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates, and for screening inhibitors. Enzyme activity may be determined using the BIOMOL GREEN Calcineurin Assay Kit (Prod. No. BML-AK804).
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