Protein components produced in E. coli. Synthesized enzymatically in vitro. (K11-only)Ubn-ubiquitinylated substrate protein was produced using K11-only mutant ubiquitin.
Purity:
High molecular weight conjugates were separated from free ubiquitin by size exclusion chromatography.
Form:
Liquid. In 50mM TRIS, pH 7.5, containing 150mM NaCl and 1mM DTT.
Target:
Ubiquitin
Application Notes:
Uses: 1. Deubiquitinylating enzyme substrates (general/linkage specific). 2. Profiling of DUB linkage-type preference or specificity in combination with other single lysine only polyubiquitinylated substrates (e.g. (K6-only)Ubn-ubiquitinylated substrate,
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