Liquid. In 20 mM Tris-HCl, pH 7.5, containing 0.5 mM DTT.
Target:
Conjugating enzyme [E2]| Conjugating enzyme [E2]
Application Notes:
Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100nM to 1µM depending upon conditions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur wit
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