Unit definition (U) (standard enzyme unit): One unit is defined as the formation of 1 µmol Neu5Ac-alpha-2,3-lactose (3SL) from 1 mM CMP-Neu5Ac and 1 mM lactose per minute at 37 C and pH 8.0. This recombinant bacterial alpha-2,3-sialyltransferase (alpha2,3SiaT) is expressed in E. coli BL21(DE3) and is designed for sialylation of glycoproteins, glycolipids, and free glycans (including oligosaccharides). It catalyzes the transfer of sialic acid from CMP-Neu5Ac (or other CMP-Neu5Ac analogues) to terminal galactose residues in a alpha-2,3 linkage. Compared to other known alpha2,3SiaTs, this enzyme has suppressed hydrolytic activities, resulting in enhanced synthesis efficiency.
Molecular Weight:
48kDa
Tag:
His-tag
Expression System:
E. coli BL21(DE3)
Purity:
90 % (SDS-PAGE)
Form:
Solid
Application Notes:
The enzyme is for research use only. Not for use in diagnostic procedures. For the sialylation of a wide range of glycans with galactose terminal: G2F, G2, Lewis a, Lewis x, lactose, LNT, LNnT, etc. For the sialylation of glycoproteins: Rituximab (in combination with beta1,4-GalT), Erythropoietin (EPO) for terminal sialylation, Fc-fusion proteins for enhanced serum half-life Glycan arrays for binding studies
alpha-2,3-sialyltransferase
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