C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[PAG-C1]
Isotype:
Mouse IgG2b
Buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage:
2°C to 8°C
Target:
PAG1
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunohistochemistry (paraffin sections): Positive tissue: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1. Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.
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