Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.
C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them it lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases, that are localized in lipid rafts, is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.
Clonality:
Monoclonal
Clone Designation:
[CSK-04]
Isotype:
Mouse IgG1
Buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage:
2°C to 8°C
Target:
CSK
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line. Western blotting: The recommended dilution of the antibody CSK-04 is 1:250-1:300, weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions. Immunoprecipitation: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates.
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