Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[AFP-11]
Isotype:
Mouse IgG1
Buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage:
2°C to 8°C
Target:
alpha-Fetoprotein
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100). Immunohistochemistry (paraffin sections): Recommended dilution: 10 µg/ml, positive tissue: hepatocellular carcinoma, heat mediated antigen retrieval (sodium citrate buffer), mAb incubation 1 hour / RT, detection DAB.Western blotting: Recommended dilution: 2 µg/ml, non-reducing conditions.
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