Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[B2M-02]
Isotype:
Mouse IgG1
Buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage:
2°C to 8°C
Target:
beta2-Microglobulin
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunohistochemistry (paraffin sections): Heat-mediated antigen retrieval, recommended dilution: 5 µg/ml, positive tissue: liver. Western blotting: Recommended dilution: 2 µg/ml, non-reducing conditions preferred. Flow cytometry: Recommended dilution: 1 µg/ml, positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes. ELISA: Working dilution should be determinated by investigator.
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