Synthetized peptide (C-terminal cytoplasmic part of STIM1).
STIM1 (stromal interacting molecule, also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[CDN3H4]
Isotype:
Mouse IgG1
Buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage:
2°C to 8°C
Target:
STIM1
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunocytochemistry: Methanol-aceton fixation, positive control: HeLa human cervix carcinoma cell line.Immunohistochemistry (paraffin sections): Recommended dilution: 5 µg/ml.Western blotting: Recommended dilution: 1 µg/ml, positive control: RBL rat basophilic leukemia cell line, both reducing and non-reducing conditions.
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