Bacterially expressed fragment of N-terminal domain of human AHNAK1.
AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear, it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[EM-09]
Isotype:
Mouse IgG1
Buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage:
2°C to 8°C
Target:
AHNAK1
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line, permeabilization is required. Western blotting: Recommended dilution: 1 µg/ml, non-reducing conditions
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