CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[323/A3]
Isotype:
Mouse IgG1
Buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage:
2°C to 8°C
Target:
CD326
Antibody Type:
Monoclonal Antibody
Application Notes:
Western blotting: Non-reducing conditions, recommended dilution: 1-2 µg/ml, positive control: MCF-7 cells. Immunoprecipitation: Recommended dilution: 1-4 µg / ml per 100-500 µg of protein in 1 ml lysate. Immunohistochemistry (paraffin sections): Recommended dilution: 1-10 µg/ml, pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37C, 1 mg / ml Tris-HCl, pH 2.0), positive tissue: breast carcinoma. Immunocytochemistry: Recommended dilution: 1-10 µg/ml. Flow cytometry: Recommended dilution: 1-4 µg/ml.
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