Dendra2 is an improved version of green-to-red photoswitchable protein Dendra, and compared to it, Dendra2 exhibits brighter fluorescence before and after photoswitching. Excitation maximum of Dendra2 is 490 nm before and 553 nm after photoactivation, and its emission maximum is 507 nm before and 573 nm after photoactivation. Activating light for Dendra2 is UV/violet to blue. Nonactivated Dendra2 spectral characteristics are similar to EGFP, and this green fluorescence can be detected at low light intensities of blue light. At hight intensities of the same blue light (or of UV/violet light) Dendra2 is photoactivated and gets emission characteristics similar to TRITC.
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[PAb 836]
Isotype:
Rabbit polyclonal
Buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Target:
Dendra2
Antibody Type:
Monoclonal Antibody
Application Notes:
Western blotting: Recommended dilution: 1-2 µg/ml. Flow cytometry: Recommended dilution: 1-4 µg/ml. Extracellular and intracellular staining.
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