STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
Clonality:
Monoclonal
Concentration:
0.1 mg/ml
Clone Designation:
[STRO-1]
Isotype:
Mouse IgM lambda
Buffer:
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage:
2°C to 8°C
Target:
STRO-1
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow cytometry: Recommended dilution: 1-5 µg/ml.
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