Chemical Name: 2,8-Dibenzyl-6-(4-hydroxyphenyl)imidazole[1,2-a]pyrazin-3(7H)-one. Alternate Names: 2-Deoxycoelenterazine
Cell viability probe
Molecular Weight:
407.46
Purity:
97% by HPLC, NMR (Conforms)
Form:
Yellow solid
CAS Number:
[50909-86-9]
Formula:
C26H21N3O2
Application Notes:
Renilla luciferase catalyzes coelentarazine oxidation by oxygen to produce light. Coelentarazine-h produces 10-fold higher light output than native coelentarazine. Coelentarazine-h also undergoes autoluminescence which is enhanced by superoxide anion and peroxynitrite in cells. May be used in gene reporter assays, live cell assays and biochemical assays (ELISA, BRET etc).
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