FAAzo4 is a photoswitchable fatty acid that cells rapidly incorporate into membrane phosphatidylcholine (PC) and phosphatidylethanolamine(PE) residues, mainly in the endoplasmic reticulum (ER). These photoswitchable membrane fatty acids allow for photoisomerization (trans-cis) leading to changes in membrane viscosity. FAAzo4-treated cells displayed altered ER export for the proteins m-Cherry-TNFalpha and EGFP-GPI. An interesting new method for manipulating membrane biophysical properties in living cells.
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