Der bunte Tobias - prestained protein ladder (6,5 - 270 Kda)

Product Overview

• Article Name: Der bunte Tobias - prestained protein ladder (6,5 - 270 Kda)
• Biozol Catalog Number: GER-GRP13284
• Supplier Catalog Number: GRP13284
• Alternative Catalog Number: GER-GRP13284
• Manufacturer: GRP – German Research Products
• Category: Molekularbiologie

Product Description

Der Bunte Tobias is a prestained protein ladder including a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa.

Additional Information

Der Bunte Tobias is a prestained protein ladder including a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recom-binant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. Der Bunte Tobias keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

 

Reaction Setup

3 μl to 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Required materials but not provided

• Vertical electrophoresis system
• Power supplies
• Vortex or equivalent
• Microcentrifuge

Guide for Molecular Weight Estimation (kDa)

Migration patterns of Der bunte Tobias Prestained Protein Ladder in different electrophoresis conditions are listed below :

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel,respectively. 2.5 μl per well for general Western transferring.

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Images

GRP13284 Migration Pattern