Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl (pH8.0) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
Expression System:
E. coli
Form:
Lyophilized powder
Sequence:
N-terminal His-Tag, Val8~Asp153
Application Notes:
Secreted frizzled-related protein 1, also known as SFRP1 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. It acts as a biphasic modulator of Wnt signaling, counteracting
GTX00065-pro Image
WB analysis of GTX00065-pro Rat SFRP1 protein (active).
Functional ELISA analysis of GTX00065-pro Rat SFRP1 protein (active) which can bind immobilized WNT2 protein.
Cell suppression effect of GTX00065-pro Rat SFRP1 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8). (A) Unstimulated HepG2 cells cultured in DMEM for 96hrs. (B) HepG2 cells cultured in DMEM, stimulated with 1000 ng/ml SFRP1 for 96hrs.
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