Human GM-CSF protein, His tag (active), Unconjugated

Product Overview

• Article Name: Human GM-CSF protein, His tag (active), Unconjugated
• Biozol Catalog Number: GTX00082-PRO
• Supplier Catalog Number: GTX00082-pro
• Alternative Catalog Number: GTX00082-PRO-10
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: FA
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: colony stimulating factor 2 , CSF , GMCSF

Product Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster

Product Properties

• NCBI: 1437
• Pubmed: 33104844,
• UniProt: P04141
• Buffer: Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl (pH8.0) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
• Expression System: HEK293 cells
• Form: Lyophilized powder
• Sequence: N-terminal His-Tag, Ala18~Glu144 (NP_000749.2)

Application Notes

• Application Notes: TF-1, the human erythroleukemia cell line, provides a good system for detecting the activity of GM-CSF for it is a cell line of immature erythroid origin that completely depends on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor

Images

GTX00082-pro Functional Assay Image

WB analysis of GTX00082-pro Human GM-CSF protein (active).

SDS-PAGE analysis of GTX00082-pro Human GM-CSF protein (active).

Cell differentiation effect of GTX00082-pro Human GM-CSF protein (active).
(A) Unstimulated U937 cells cultured in RPMI-1640.
(B) U937 cells cultured in RPMI-1640, stimulated with GM-CSF (10 ng/ml).