Human NGF protein, His tag (active), Unconjugated

Catalog Number: GTX00097-PRO
Article Name: Human NGF protein, His tag (active), Unconjugated
Biozol Catalog Number: GTX00097-PRO
Supplier Catalog Number: GTX00097-pro
Alternative Catalog Number: GTX00097-PRO-10
Manufacturer: GeneTex
Category: Proteine/Peptide
Application: FA
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: nerve growth factor , Beta-NGF , HSAN5 , NGFB
This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the
NCBI: 4803
UniProt: P01138
Buffer: Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl (pH8.0) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
Expression System: E. coli
Form: Lyophilized powder
Sequence: N-terminal His-Tag, Glu19~Arg121+DDDDK+Ser122~Arg239 (NP_002497.2)
Application Notes: Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. As reported, when the pheochromocytoma cell line PC12 is exposed to ne
GTX00097-pro Image
Cell differentiation effect of GTX00097-pro Human NGF protein (active).
(A) Unstimulated PC-12 cells cultured in Ham's F12K containing 5% fetal calf serum and 10% horse serum on collagen coated plates.
(B) PC-12 cells cultured in Ham's F12K containing 5% fetal calf serum and 10% horse serum on collagen coated plates, stimulated with 10 ng/ml NGF for 6 days.
SDS-PAGE analysis of GTX00097-pro Human NGF protein (active).
Functional ELISA analysis of GTX00097-pro Human NGF protein (active) which can bind immobilized a2M protein.