Human TNF alpha protein, His tag (active), Unconjugated

Product Overview

• Article Name: Human TNF alpha protein, His tag (active), Unconjugated
• Biozol Catalog Number: GTX00102-PRO
• Supplier Catalog Number: GTX00102-pro
• Alternative Catalog Number: GTX00102-PRO-10
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: FA
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F

Product Description

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1

Product Properties

• NCBI: 7124
• UniProt: P01375
• Buffer: Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl (pH8.0) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
• Expression System: E. coli
• Form: Lyophilized powder
• Sequence: N-terminal His-Tag, Val77~Leu233 (NP_000585.2)

Application Notes

• Application Notes: TNF-a (Tumor necrosis factor), is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It has been reported that TNFa can inhibit the proliferation and induce apoptosis of A549 cells, besides, the concentration of IL-1beta a

Images

GTX00102-pro Functional Assay Image

SDS-PAGE analysis of GTX00102-pro Human TNF alpha protein (active).

WB analysis of GTX00102-pro Human TNF alpha protein (active).

ELISA detection of secreted IL-1 beta activation from A549 cells induced by GTX00102-pro Human TNF alpha protein (active) for 8hrs.
Recombinant protein concentrations: 1, 10, 100, and 1000 ng/ml.