Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
Expression System:
E. coli
Form:
Lyophilized powder
Sequence:
N-terminal His-Tag, Pro36~Leu205 (NP_000586.2)
Application Notes:
TNF-beta, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. The biological effects of TNF-beta are very similar to TNF-alpha, due to the similarity of molecular structure and the receptors. As reported, TNF-beta could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1beta in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNF-beta (10 ng/ml) for 8h, 24h, 48h, 72h, then cells were observed by inverted microscope.
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