Human IL17 Receptor C protein, His tag, Unconjugated

Product Overview

• Article Name: Human IL17 Receptor C protein, His tag, Unconjugated
• Biozol Catalog Number: GTX00143-PRO
• Supplier Catalog Number: GTX00143-pro
• Alternative Catalog Number: GTX00143-PRO-10
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: FA
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: interleukin 17 receptor C , CANDF9 , IL17-RL , IL17RL

Product Properties

• NCBI: 84818
• UniProt: Q8NAC3
• Buffer: Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4), 0.01% SKL, 1mM DTT, 5% Trehalose, ProClin 300.
• Expression System: E. coli
• Form: Lyophilized powder
• Sequence: N-terminal His-Tag, Asn257~Asn477 (NP_001190192.1)

Application Notes

• Application Notes: IL17RC (Interleukin-17 receptor C) is a receptor for IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RA. This protein is expressed in nonhemopoietic tissues, and binds both IL-17A and IL-17F with similar affinities. A binding ELISA assay was conducted to detect the association of IL17RC with IL17RA. Briefly, IL17RC were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-IL17RC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of IL17RC with IL17RA was in a dose dependent manner.