Human CLCA1 protein, His tag, Unconjugated
Catalog Number:
GTX00147-PRO
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| Article Name: | Human CLCA1 protein, His tag, Unconjugated |
| Biozol Catalog Number: | GTX00147-PRO |
| Supplier Catalog Number: | GTX00147-pro |
| Alternative Catalog Number: | GTX00147-PRO-10 |
| Manufacturer: | GeneTex |
| Category: | Proteine/Peptide |
| Application: | FA |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | chloride channel accessory 1 , CACC , CACC1 , CLCRG1 , CaCC-1 , GOB5 , hCLCA1 , hCaCC-1 |
| Application Notes: | CLCA1 (Calcium-activated chloride channel regulator 1) is a member of the calcium sensitive chloride conductance protein family. This protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits. It has been reported that CLCA1 activates calcium-dependent chloride channel through the interaction with TMEM16A (anoctamin-1). Besides, there exits similarities between human and mouse TMEM16A in amino acid sequence with the identity of 89.66% . Thus, a functional ELISA assay was conducted to detect the association of recombinant human CLCA1 with recombinant mouse TMEM16A. Briefly, CLCA1 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to TMEM16A-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-CLCA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of CLCA1 with TMEM16A was in a dose dependent manner. |
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