Human VEGF Receptor 2 protein, His tag (active), Unconjugated
Catalog Number:
GTX00259-PRO
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| Article Name: | Human VEGF Receptor 2 protein, His tag (active), Unconjugated |
| Biozol Catalog Number: | GTX00259-PRO |
| Supplier Catalog Number: | GTX00259-pro |
| Alternative Catalog Number: | GTX00259-PRO-10 |
| Manufacturer: | GeneTex |
| Category: | Proteine/Peptide |
| Application: | FA |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | kinase insert domain receptor , CD309 , FLK1 , VEGFR , VEGFR2 |
| Application Notes: | Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) also known as kinase insert domain receptor acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. VEGFR2 functions as the primary mediator of vascular endothelial growth factor activation in endothelial cells. regulation of VEGFR-2 expression appears critical in mitogenesis, differentiation, and angiogenesis. To test the effect on inhibit the VEGF-dependent proliferation of endothelium cells, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1µg/ml Vascular Endothelial Growth Factor C (VEGFC) and various concentrations of recombinant human VEGFR2. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37C. Proliferation of ECV-304 cells after incubation with VEGFR2 for 96h observed by inverted microscope. |
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