Reconstitute with 100 mM NaHCO3 and 500 mM NaCl (pH8.3) to 0.1-1.0 mg/mL. Do not vortex. Lyophilized from 100 mM NaHCO3 (pH 8.3) with 500 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
Expression System:
E. coli
Form:
Lyophilized powder
Sequence:
N-terminal His-Tag, Asp23~Phe235 (NP_001257932.1)
Application Notes:
Tissue Factor Pathway Inhibitor 2 (TFPI2) takes part in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. TFPI2 does not have any influence on thrombin. TFPI2 also can inhibit MMP activity, which can hydrolyze gelatin under certain conditions. Thus, the activity of TFPI2 can be measured by inhibit MMP-2 hydrolyze gelatin. Gelatin zymography is mainly used for the detection of the gelatinases, 2µg/ml was denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate-polyacrylamide gel (SDS-PAGE, 8% gels) containing gelatin (1 mg/ml) with nonreducing conditions. After renaturation, incubate with various concentrations of recombinant human TFPI2, then staining with coomassie brilliant blue G250, active MMP-2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel, if the activity of MMP-2 inhibit by TFPI2, there was none white binds on the gel. MMP-2 can be inhibited by recombinant human TFPI2 at least 5µg/ml.
* VAT and and shipping costs not included. Errors and price changes excepted
