Mouse Interferon gamma protein, His tag (active), Unconjugated
Biozol Catalog Number:
GTX00283-PRO
Supplier Catalog Number:
GTX00283-pro
Alternative Catalog Number:
GTX00283-PRO-10
Manufacturer:
GeneTex
Category:
Proteine/Peptide
Application:
FA
Species Reactivity:
Mouse
Conjugation:
Unconjugated
Alternative Names:
interferon gamma , IFN-g , Ifg
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor
Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl (pH8.0) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
Expression System:
E. coli
Form:
Lyophilized powder
Sequence:
N-terminal His-Tag, His23~Cys155 (NP_032363.1)
Application Notes:
IFN-gamma is an important activator of macrophages, it promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages. After stimulated with IFN-gamma, morphological changes will occur in murine macrophage cell line (Raw 246. 7 cells), and inducibl
GTX00283-pro Image
Activation effect of GTX00283-pro Mouse Interferon gamma protein (active). (A) Unstimulated Raw 264.7 cells cultured in DMEM. (B) Raw 264.7 cells cultured in DMEM, stimulated with IFN gamma.
SDS-PAGE analysis of GTX00283-pro Mouse Interferon gamma protein (active).
ELISA detection of iNOS protein expression from RAW 246.7 cells stimulated by GTX00283-pro Mouse Interferon gamma protein (active) at 2 ng/ml for 24hrs.
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