Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl (pH8.0) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
Expression System:
HEK293 cells
Form:
Lyophilized powder
Sequence:
N-terminal His-Tag, Ala21~Gln169 (NP_032392.1)
Application Notes:
Interleukin-2 (IL-2), the main lymphokine produced by activated CD4 T-helper lymphocytes, is a potent T-cell growth regulator. In addition to this activity, IL-2 was found to decrease the proliferation of 3T3 fibroblasts in time- and dose-dependent manne
GTX00285-pro Image
WB analysis of GTX00285-pro Mouse IL2 protein (active).
The dose effect curve of GTX00285-pro Mouse IL2 protein (active) on 3T3 cells. After incubation of Mouse IL2 protein (active) for 48 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).
The inhibition effect of GTX00285-pro Mouse IL2 protein (active) on cell proliferation. (A) Unstimulated 3T3 cells cultured in DMEM for 72hrs. (B) 3T3 cells cultured in DMEM, stimulated with IL2 (10 ng/ml) for 72hrs.
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