Mouse TNF alpha protein, His tag (active), Unconjugated

Product Overview

• Article Name: Mouse TNF alpha protein, His tag (active), Unconjugated
• Biozol Catalog Number: GTX00287-PRO
• Supplier Catalog Number: GTX00287-pro
• Alternative Catalog Number: GTX00287-PRO-10
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: FA
• Species Reactivity: Mouse
• Conjugation: Unconjugated
• Alternative Names: tumor necrosis factor , DIF , TNF-a , TNF-alpha , TNFSF2 , TNFalpha , Tnfa , Tnfsf1a , Tnlg1f

Product Description

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II t

Product Properties

• NCBI: 21926
• UniProt: P06804
• Buffer: Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl (pH8.0) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
• Expression System: E. coli
• Form: Lyophilized powder
• Sequence: N-terminal His-Tag, Leu80~Leu235 (NP_001265530.1)

Application Notes

• Application Notes: TNF-a (Tumor necrosis factor), being an endogenous pyrogen, is able to induce fever, apoptotic cell death, inflammation and inhibit tumorigenesis. As reported, TNFa could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration

Images

GTX00287-pro Functional Assay Image

SDS-PAGE analysis of GTX00287-pro Mouse TNF alpha protein (active).

WB analysis of GTX00287-pro Mouse TNF alpha protein (active).

ELISA detection of secreted IL-1 beta expression from A549 cells stimulated by GTX00287-pro Mouse TNF alpha protein (active) at 1 ng/ml or 10 ng/ml for 48hrs.