Mouse LCAT protein, His tag, Unconjugated

Product Overview

• Article Name: Mouse LCAT protein, His tag, Unconjugated
• Biozol Catalog Number: GTX00300-PRO
• Supplier Catalog Number: GTX00300-pro
• Alternative Catalog Number: GTX00300-PRO-10
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: FA
• Species Reactivity: Mouse
• Conjugation: Unconjugated
• Alternative Names: lecithin cholesterol acyltransferase , AI046659 , D8Wsu61e

Product Properties

• NCBI: 16816
• UniProt: P16301
• Buffer: Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4), 0.01% SKL, 1mM DTT, 5% Trehalose, ProClin 300.
• Expression System: E. coli
• Form: Lyophilized powder
• Sequence: N-terminal His-Tag, Glu41~His210 (NP_032516.2)

Application Notes

• Application Notes: LCAT (Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that APOA1 (Apolipoprotein A-I) acts as a cofactor for the LCAT. Thus, a binding ELISA assay was conducted to detect the association of LCAT with APOA1. Briefly, recombinant rat LCAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to APOA1-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with APOA1 was in a dose dependent manner.